Contractile Skeletal Muscle Microtissues in Microchannel

This paper reports a development of the microfluidic device for fabrication and cultivation of contractile skeletal muscle tissue in microchannels. Microfluidic processes were only used to develop contractile skeletal muscle microtissues in microchannels. Thus, the volume of the collagen gel used to fabricate the microtissues can be defined precisely. The fabricated microtissues can be cultured under a more in vivo-like environment and used for higher throughput applications. Tunnel-like microchannels connecting 2 microchannels realized to pattern the collagen gel with cells and supply the medium to the cells (Fig.1). The developed microtissues in microchannels may be powerful tools for drug development and biological research.